Journal: Biology

Article Title: Shoseiryuto May Prevent Bronchial Epithelial Tight Junction Disruption by Inhibiting the Inflammatory NF-κB Signaling Pathway

doi: 10.3390/biology15080603

Figure Lengend Snippet: Effects of NF-κB signaling inhibitors SC-514 and BAY11-7085 on Poly I:C-induced inflammation and TJ barrier disruption. ( a , b ) Inflammatory markers: IL-6 medium concentration and IL-6 mRNA expression. ( c – f ) TJ barrier markers: TEER, Na–F permeability, occludin mRNA expression, and immunofluorescent-stained protein. Occludin is shown in red and nuclei are stained with DAPI (blue). Data are presented as mean ± SD ( n = 3). ** p < 0.01, *** p < 0.001 versus Ctrl; † p < 0.05, †† p < 0.01, ††† p < 0.001 versus Poly I:C (one-way ANOVA with Dunnett’s test).

Article Snippet: LPS and H 2 O 2 were sourced from Sigma-Aldrich (St. Louis, MO, USA); TNF-α from Fujifilm Wako (Osaka, Japan); Poly I:C from InvivoGen (San Diego, CA, USA); and the NF-κB inhibitors SC-514 and BAY11-7085 from Abcam (Cambridge, UK) and TargetMol (Boston, MA, USA), respectively.

Techniques: Disruption, Concentration Assay, Expressing, Permeability, Staining

Journal: Biology

Article Title: Shoseiryuto May Prevent Bronchial Epithelial Tight Junction Disruption by Inhibiting the Inflammatory NF-κB Signaling Pathway

doi: 10.3390/biology15080603

Figure Lengend Snippet: Effects of SST and BAY11-7085 on Poly I:C-induced NF-κB signaling activity. Concentrations: SST 0.3 mg/mL, BAY11-7085 (BAY) 5 μM, and Poly I:C (Poly) 10 μg/mL. ( a , b ) NF-κB (p65) and phosphorylated p65 (P-p65) proteins; ( c ) phosphorylation ratio (P-p65/p65); ( d , e ) IκBα (IκB) and phosphorylated IκBα (P-IκB) proteins; ( f ) phosphorylation ratio (P-IκB/IκB). Data are presented as mean ± SD ( n = 3). * p < 0.05, *** p < 0.001 versus Ctrl; † p < 0.05, ††† p < 0.001 versus Poly (one-way ANOVA with Dunnett’s test). The original, untrimmed Western blot images ( a , b , d , e ) are shown in the .

Article Snippet: LPS and H 2 O 2 were sourced from Sigma-Aldrich (St. Louis, MO, USA); TNF-α from Fujifilm Wako (Osaka, Japan); Poly I:C from InvivoGen (San Diego, CA, USA); and the NF-κB inhibitors SC-514 and BAY11-7085 from Abcam (Cambridge, UK) and TargetMol (Boston, MA, USA), respectively.

Techniques: Activity Assay, Phospho-proteomics, Western Blot